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characterization of expressed sequence tags from a gallus gallus pineal gland cdna library特征的表达序列标签背带吊裤带松果体cdna图书馆
Comparative and Functional Genomics
Comp Funct Genom 2005; 6: 301–306.
Published online 11 July 2005 in Wiley InterScience (). DOI: 10.1002/cfg.484
Research Article
Characterization of expressed sequence tags
from a Gallus gallus pineal gland cDNA library
1 1 1 1 2 1
Stefanie Hartman , Greg Touchton , Jessica Wynn , Tuoyu Geng , Nelson W. Chong and Ed Smith *
1Comparative Genomics Lab, Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA 24061, USA
2 Division of Cardiology, Department of Medicine, University of Leicester, Leicester, UK
*Correspondence to: Abstract
Ed Smith, 2250 Litton Reaves
Hall, Blacksburg, VA The pineal gland is the circadian oscillator in the chicken, regulating diverse
24061, USA. functions ranging from egg laying to feeding. Here, we describe the isolation and
E-mail: esmith@ characterization of expressed sequence tags (ESTs) isolated from a chicken pineal
gland cDNA library. A total of 192 unique sequences were analysed and submitted
to GenBank; 6% of the ESTs matched neither GenBank cDNA sequences nor the
newly assembled chicken genomic DNA sequence, three ESTs aligned with sequences
designated to be on the Z random, while one matched a W chromosome sequence and
could be useful in cataloguing functionally important genes on this sex chromosome.
Additionally, single nucleotide polymorphisms (SNPs) were identified and validated
in 10 ESTs tha
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