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chromatographic separation and characterization of capsaicinoids and their analogues色谱分离和鉴定辣椒素及其类似物.pdf

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chromatographic separation and characterization of capsaicinoids and their analogues色谱分离和鉴定辣椒素及其类似物

Pharmacology Pharmacy, 2011, 2, 136-140 doi:10.4236/pp.2011.23018 Published Online July 2011 (/journal/pp) Chromatographic Separation and Characterization of Capsaicinoids and Their * Analogues 1 2 2 3 4 5 Junlian Wang , Li Zhou , Lei Wang , Zhenghong Peng , Shengze Zhou , Xiangfeng Zhou , Xiaobin Zhang1, Bixian Peng6# 1Department of Materials Science and Engineering, Zhejiang University, Hangzhou, China; 2Chenguang Engineering Technology Research Center of Natural Pigments, Hebei, China; 3Beijing TianGongLongYuan International Biochemical Company, Beijing, China; 4Beijing JiuZhouLongLing Environmental Protection Material Company, Beijing, China; 5Institute of Chemistry, Chinese Academy of Sciences (CAS), Beijing, China; 6Technical Institute of Physics and Chemistry, CAS, Beijing, China. Email: #pengbx@ Received December 19th, 2010; revised May 4th, 2011; accepted May 15th, 2011. ABSTRACT An attempt was made to establish a chromatographic separation and analysis method for capsaicinoids and their ana- logues. A series of factors,such as choice of column and eluents, eluent composition,flow rate of eluent and operational temperature were correlated, countereacted and optimized to attain appropriate separation efficiency and finalize the experimentally oprational procedures as a whole, permiting a mixture comprising 8 capsaicinoids including analogues to be separated and Characterized with an allowed deviations and within a period of 36 minutes via 1 test as well. Keywords : Capsaicinoid, Chromatography, Separation, Characterization 1. Introduction medical researchers in deed. In order to bolster such a