瘢痕疙瘩p53基因检测试剂盒临床应用.doc

瘢痕疙瘩p53基因检测试剂盒临床应用[摘要]目的：在浙江地区对瘢痕疙瘩p53 基因检测试剂盒进行临床实验, 评价此试剂盒的准确度和有效性。方法：采用聚合酶链反应-反向点杂交方法建立的试剂盒检测了浙江地区75例瘢痕疙瘩患者与75例正常对照的p53基因第72位密码子多态性位点的基因型。结果：瘢痕疙瘩组的Pro等位基因及Pro/Pro基因型频率明显高于对照组(P值分别为0.014、0.015)，Pro/Pro基因型者患瘢痕疙瘩的风险性明显增高(OR=2.404,95%CI:1.182～4.887)。结论：p53基因检测试剂盒可以预测瘢痕疙瘩高危个体，对于临床诊断和治疗方面也有一定的指导意义，并具有良好的可重复性和特异性。 [关键词]瘢痕疙瘩；p53基因；基因多态性检测；试剂盒 [中图分类号]Q813.1[文献标识码]A[文章编号]1008-6455（2008）05-03 The application of p53 gene detection kit for suscepbility of keloid ZHUO Yang,GAO Jian-hua,ZENG Xing-ye,HUANG Da-dao,PAN Ruo-wang,CAO Xiao-en (Department of Surgery,the 118th Hospital of PLA,Wenzhou 325000,Zhejiang,China) Abstract： ObjectiveTo investigate the keloid testing box of p53 gene by clinic experiment and judge its validity and accuracy in Zhejiang Province,China.MethodsThe p53 genotypes were determined by polymerase chain reaction-reverse dot blot(PCR-RDB) that the kit adopt among 75 patients with keloid and 75 unrelated healthy controls from Zhejiang Province.ResultsThe frequency of the p53 Pro allele and the Pro/Pro genotype among keloid patients was significantly higher than that among healthy controls(P=0.014、0.015 respectively).The p53 Pro/Pro genotype significantly increased the risk for developing keloid,compared to the combination of Pro/Arg and Arg/Arg genotypes,with the odds ratio(OR) of 2.404(95%CI: 1.182～4.887).ConclusionThe p53 gene detection kit can be used to predicate high-risk individuals for keloid,it is useful to clinical diagnose and therapy effects monitoring.The keloid testing box is specific and valuable to repeated detection. Key words：keloid;p53 gene;gene polymorphism detection;reagent kit 瘢痕疙瘩属于病理性瘢痕，是由于结缔组织对创伤的过度增生反应而形成。研究结果提示瘢痕疙瘩成纤维细胞的过量增生和凋亡相对减少在瘢痕疙瘩的发生发展中起着重要作用[1]。p53基因是重要的细胞周期调节、凋亡基因，在多种肿瘤细胞中均可发现p53蛋白的聚集现象,并与p53基因突变或缺失有关[2]。随着分子遗传学的进展，了解遗传因素在疾病中的作用将有利于疾病的诊断、治疗和预防,也有助于了解环境因素在疾病发生中的作用。我们的前期工作已发现p53基因第72密码子多态性与瘢痕疙瘩的发生存在关联关系，在此,我们在浙江地区应用此试剂盒对瘢痕疙瘩临床患者做相关检查, 检验其有效性, 为临床瘢痕疙瘩的预防和治疗提供可靠的途径。 1材料和方法 1.1 试剂盒组成（一个试剂盒可检测10份样品）