comparison between dna immobilization techniques on a redox polymer matrix比较dna固定技术在氧化还原聚合物矩阵.pdfVIP

American Journal of Analytical Chemistry , 2011, 2, 392-400 doi:10.4236 /ajac.2011.23048 Published Online July 20 11 (/journal/ajac) Comparison Between DNA Immobilization Techniques on a Redox Polymer Matrix 1 1* 2 2 3 Vijayalakshmi Velusamy , Khalil Arshak , Catherine F. Yang , Lei Yu , Olga Korostynska , Catherine Adley4 1 Electronic and Computer Engineering Department, University of Limerick, Limerick, Ireland 2Department of Chemistry and Biochemistry , Rowan University, Glassboro, USA 3School of Physics, Dublin Institute of Technology, Dublin , Ireland 4Department of Chemical and Environmental Sciences, University of Limerick, Limerick , Ireland E-mail: viji.subha@ul.ie Received February 16, 2011; revised April 2, 2011; accepted April 16, 2011 Abstract In this paper we report a label-free detection method of unmodified DNA using polypyrrole as an immobili- zation matrix by impedance measurement. A probe and a target complementary DNA sequence specific for the bacterial pathogen, Bacillus cereus are used. Impedance measurements are performed without using ad- ditional redox probes. The effects of hybridization and non-specific binding are compared when the Probe DNA molecules were immobilized by two different methods: electrochemical adsorption and entrapment. The probe DNA immobilized using electrochemical adsorption yielded better hybridization signals com- pared to that immobilized using the entrapment method. Control experiments were also performed to prove the specificity of the biosensor in the presence of non complementary