control of aptamer function using radiofrequency magnetic field使用射频磁场控制适配子函数.pdfVIP

SAGE-Hindawi Access to Research Journal of Nucleic Acids Volume 2011, Article ID 103872, 6 pages doi:10.4061/2011/103872 Research Article Control of Aptamer Function Using Radiofrequency Magnetic Field Kenichi Taira,1 Koichi Abe,1 Takayuki Ishibasi,2, 3 Katsuaki Sato,2, 4 and Kazunori Ikebukuro1 1 Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan 2 Department of Applied Physics, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan 3 Department of Material Science and Technology, Nagaoka University of Technology 1603-1, Kamitomioka, Nagaoka, Niigata 940-2188, Japan 4 PRESTO Program, Japan Science and Technology Agency, 5 Sambancho, Chiyodaku, Tokyo 102-0075, Japan Correspondence should be addressed to Kazunori Ikebukuro, ikebu@cc.tuat.ac.jp Received 22 May 2011; Accepted 28 June 2011 Academic Editor: Daisuke Miyoshi Copyright © 2011 Kenichi Taira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Remote control of aptamer function has allowed us to control protein function in space and time. Here, we propose a novel control system for aptamer function by radiofrequency magnetic field- (RFMF-) induced local heating of a gold nanoparticle conjugated with an aptamer. In this study, we used a 31-mer thrombin-binding aptamer (TBA), which can inhibit thrombin activity, as a model aptamer. We evaluated the RFMF control of the inhibitory activity of a gold nanoparticle-conjugated TBA. To evaluate the effect of RFMF on enzymatic activity, we utilized a complem