原创力文档conversion of carbon dioxide to metabolites by clostridium acetobutylicum kctc1037 cultivated with electrochemical reducing power二氧化碳转换成由梭状芽胞杆菌代谢物acetobutylicum kctc1037与电化学还原能力培养.pdfVIP
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conversion of carbon dioxide to metabolites by clostridium acetobutylicum kctc1037 cultivated with electrochemical reducing power二氧化碳转换成由梭状芽胞杆菌代谢物acetobutylicum kctc1037与电化学还原能力培养
Advances in Microbiology, 2012, 2, 332-339
/10.4236/aim.2012.23040 Published Online September 2012 (http://www.SciRP.org/journal/aim)
Conversion of Carbon Dioxide to Metabolites by
Clostridium acetobutylicum KCTC1037 Cultivated with
Electrochemical Reducing Power
1 2 1*
Bo Young Jeon , Il Lae Jung , Doo Hyun Park
1Department of Biological Engineering, Seokyeong University, Seoul, South Korea
2Department of Radiation Biology, Environmental Radiation Research Group,
Korea Atomic Energy Research Institute, Daejeon, South Korea
*
Email: baakdoo@skuniv.ac.kr
Received June 21, 2012; revised July 29, 2012; accepted August 6, 2012
ABSTRACT
In this research, metabolic fixation of CO by growing cells of C. acetobutylicum cultivated with electrochemical re-
2
ducing power was tested on the basis of the metabolites production and genes expression. In cyclic voltammetry, elec-
trochemical oxidation and reduction reaction of neutral red (NR) immobilized in intact cells of C. acetobutylicum was
stationarily repeated like the soluble one in the condition without CO2 but the electrochemical reduction reaction was
selectively increased by addition of CO2 . In electrochemical bioreactor, the modified graphite felt cathode with NR
(NR-cathode) induced C. acetobutylicum to generate acetate, propionate, and butyrate from CO2 in defined medium.
When H and CO were used as an electron donor and an electron acceptor, respectively,
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