databasing molecular identities of sugarcane (saccharum spp.) clones constructed with microsatellite (ssr) dna markers物品清单分子身份的蔗糖(蔗糖spp)。.pdfVIP

American Journal of Plant Sciences , 2010, 1, 87-94 87 doi:10.4236/ajps.2010.12011 Published Online December 2010 (http://www.SciRP.org/journal/ajps) Databasing Molecular Identities of Sugarcane (Saccharum spp.) Clones Constructed with Microsatellite (SSR) DNA Markers Yong-Bao Pan Sugarcane Research Laboratory, Houma, USA. Email: yongbao.pan@ Received October 1st st th , 2010; revised October 31 , 2010; accepted November 13 , 2010. ABSTRACT This paper reports the development of the first SSR marker-based sugarcane (Saccharum spp.) molecular identity da- tabase in the world. Since 2005, 1,025 sugarcane clones were genotyped, including 811 Louisiana, 45 Florida, 39 Texas, 130 foreign, and eight consultant/seed company clones. Genotyping was done on a fluorescence-capillary elec- trophoresis detection platform involving 21 highly polymorphic SSR markers that could potentially amplify 144 distinc- tive DNA fragments. Genotyping data were processed with the GeneMapper™ software to reveal electrophoregrams that were manually checked against the 144 fragments. The presence (A) or absence (C) of these 144 fragments in any sugarcane clone was recorded in an affixed sequence order as a DNAMAN® file to represent its molecular identity be- ing achieved into a local molecular identity database. The molecular identity database has been updated annually by continued genotyping of newly assigned sugarcane clones. The database provides molecular descriptions for new cul- tivar registration articles, enables sugarcane breeders to identify mis-labeled sugarcane clones in crossing programs and determine the paternity of cross progeny, and ensures the desired cultivars are grown in farmers’ fields. Keywords : Sugarcane (Saccharum spp.), Breeding, SSR Marker, Mole