detection of thyroid carcinoma antigen with quantum dots and monoclonal igm antibody (jt-95) system用量子点检测甲状腺癌抗原和单克隆igm抗体(jt - 95)系统.pdfVIP

Hindawi Publishing Corporation Journal of Nanomaterials Volume 2010, Article ID 937684, 7 pages doi:10.1155/2010/937684 Research Article Detection of Thyroid Carcinoma Antigen with Quantum Dots and Monoclonal IgM Antibody (JT-95) System Kouki Fujioka,1, 2 Noriyoshi Manabe,2 Mayumi Nomura,1 Michiko Watanabe,1 Hiroshi Takeyama,3 Akiyoshi Hoshino,1, 2 Sanshiro Hanada,2 Kenji Yamamoto,2 and Yoshinobu Manome1 1 Department of Molecular Cell Biology, Institute of DNA Medicine, Jikei University School of Medicine, 3-25-8 Nishi-Shinbashi, Minato, Tokyo 105-8461, Japan 2 International Clinical Research Center, Research Institute, International Medical Center of Japan, 1-21-1 Toyama, Shinjuku, Tokyo 162-8655, Japan 3 Department of Surgery, Jikei University School of Medicine, 4-11-1 Izumi-Honcho, Komae City, Tokyo 201-8601, Japan Correspondence should be addressed to Yoshinobu Manome, manome@jikei.ac.jp Received 1 October 2009; Revised 3 December 2009; Accepted 8 March 2010 Academic Editor: Chao Lin Copyright © 2010 Kouki Fujioka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. High-intensity fluorescent nanoparticles, quantum dots (QDs), have been applied to a wide range of biological studies and medical studies by taking advantage of their fluorescent properties. On the other hand, we have reported the specificity of JT-95 monoclonal IgM antibody, which recognizes the antigen of thyroid carcinomas. Here we show that the combination of QDs and JT-95 monoclonal antibody was applicable to Western blotting analysis, ELISA-like system, and fluorescent microscopic analysis of S