distinct subcellular localization of gsk-3β in melanocytic nevi implications in melanocyte senescence不同的亚细胞定位的gsk-3βmelanocytic痣在黑素细胞衰老的影响.pdfVIP

Open Journal of Pathology, 2012, 2, 113-119 1 Published Online October 2012 (http://www.SciRP.org/journal/ojpathology) /10.4236/ojpathology.2012.24021 Distinct Subcellular Localization of GSK-3β in Melanocytic * Nevi: Implications in Melanocyte Senescence 1# 1 2 1 1 Jonathan L. Curry , Carlos A. Torres-Cabala , Carla L. Warneke , Peter Zhang , Victor G. Prieto 1Departments of Pathology and Dermatology, The University of Texas MD Anderson Cancer Center, Houston, USA; 2Departments of Biostatistics, The University of Texas MD Anderson Cancer Center, Houston, USA. # Email: jlcurry@ th st rd Received July 25 , 2012; revised August 21 , 2012; accepted September 3 , 2012 ABSTRACT Melanocytic nevi are a transient in vivo proliferation of melanocytes that after time undergo cellular senescence. Most nevi harbor B-Raf mutations, which appear to activate cellular mechanisms of senescence in melanocytes. Glycogen synthase kinase 3β (GSK-3β), a critical downstream effector of the AKT signaling pathway, is involved in the devel- opment of melanoma and has been associated with senescence in melanocytes. Our immunohistochemical and im- munofluorescence studies revealed distinct, perinuclear, dot-like reactivity of GSK-3β in melanocytic nevi. Furthermore, our tissue microarray analysis demonstrated significant perinuclear dot-like sublocalization of GSK-3β in melanocytic nevi compared with the amount of GSK-3β observed in melanoma (P 0.0019). In summary, the subcellular localiza- tion of GSK-3β