early gene expression in wounded human keratinocytes revealed by dna microarray analysis早期基因表达在人类角质细胞受伤了dna微阵列分析.pdfVIP

early gene expression in wounded human keratinocytes revealed by dna microarray analysis早期基因表达在人类角质细胞受伤了dna微阵列分析.pdf

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early gene expression in wounded human keratinocytes revealed by dna microarray analysis早期基因表达在人类角质细胞受伤了dna微阵列分析

Comparative and Functional Genomics Comp Funct Genom 2003; 4: 47–55. Published online in Wiley InterScience (). DOI: 10.1002/cfg.239 Conference Paper Early gene expression in wounded human keratinocytes revealed by DNA microarray analysis 1 ` 1 2 1 3 Manal A. Dayem , Chimene Moreilhon , Laurent Turchi , Virginie Magnone , Richard Christen , Gilles Ponzio2 1 and Pascal Barbry * 1 ´ Laboratoire de Physiologie Genomique des Eucaryotes, CNRS/UNSA UMR 6097, IPMC, F-06560 Sophia Antipolis, France 2 ´ ´ Laboratoire de Biologie et Physiologie de la Peau, INSERM U385, Faculte de Medecine, F-06107 Nice, France 3Laboratoire de Physiologie des Membranes Cellulaires, CNRS UMR6078 Bat. J. Maetz, Villefranche-sur-Mer, France *Correspondence to: Abstract Pascal Barbry, Laboratoire de ´ Wound healing involves several steps: spreading of the cells, migration and prolifer- Physiologie Genomique des Eucaryotes, IPMC, 660, Route ation. We have profiled gene expression during the early events of wound healing in des Lucioles, Sophia Antipolis, normal human keratinocytes with a home-made DNA microarray containing about 06560 Valbonne, France. 1000 relevant human probes. An original wounding machine was used, that allows the E-mail: barbry@rs.fr wounding of up to 40% of the surface of a confluent monolayer of cultured cells grown on a Petri dish (compared with 5% with a classical ‘scratch’ method). The two aims of the present study were: (a) to validate a limited num

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