REM睡眠剥夺对大鼠恐惧消退再现影响.pdf

照组大鼠放回鼠笼，训练6 h 后对6-12 h RSD 组大鼠进行REM 睡眠剥夺；第四天在 场景A 中进行恐惧消退再现检测20 min （无任何刺激呈现）。研究表明实验一和实 验二两种任务不依赖于海马，实验三和实验四两种任务是海马依赖性的。在恐惧条件 化、恐惧消退训练及消退再现检测阶段记录分析大鼠的僵直行为。 结果：实验一：在恐惧条件化以及恐惧消退训练阶段，0-6 h RSD 组、6-12 h RSD 组与各自对照组大鼠的僵直水平组间差异都无显著性；在恐惧消退再现检测阶段，0-6 h RSD 组大鼠的僵直水平显著高于对照组，6-12 h RSD 组与对照组大鼠的僵直水平组 间差异无显著性。实验二：在恐惧条件化以及恐惧消退训练阶段，0-6 h RSD 组、6-12 h RSD 组与各自对照组大鼠的僵直水平组间差异都无显著性；在恐惧消退再现检测阶 段，0-6 h RSD 组大鼠的僵直水平显著高于对照组，6-12 h RSD 组与对照组大鼠的僵 直水平组间差异无显著性。实验三：在恐惧条件化、恐惧消退训练以及恐惧消退再现 检测阶段，0-6 h RSD 组、6-12 h RSD 组与各自对照组大鼠的僵直水平组间差异都无 显著性。实验四：在恐惧条件化、恐惧消退训练以及恐惧消退再现检测阶段，0-6 h RSD 组、6-12 h RSD 组与各自对照组大鼠的僵直水平组间差异都无显著性。 结论：恐惧消退训练后的RSD 对于恐惧消退记忆的影响是复杂的，RSD 损害海 马非依赖性恐惧消退再现，而对海马依赖性任务没有影响，并且这种损害依赖于恐惧 消退训练后睡眠剥夺的时段，进一步说明REM 睡眠选择性参与特定任务的记忆过程。 关键词：睡眠剥夺，恐惧消退，海马，场景，僵直，大鼠 4 Effects of REM sleep deprivation on recall of fear extinction in rats ABSTRACT Objectives ：The present study was to examine the effects of post-extinction rapid eye movement sleep deprivation(RSD) on recall of fear extinction。 Methods：Experiment 1：On Day 1，rats were transported to context A to habituate them to the context；On Day 2(fear conditioning phase)， Rats received five pairings of tone and footshock in context A ；The next day(extinction training phase) ，rats received ten tone-alone extinction trials in context A，immediately，0-6 h RSD rats were placed in the RSD apparatus and 6-12 h RSD rats and control rats were placed in their homecages，six hours later，6-12 h RSD rats were placed in the RSD apparatus；On Day 4(recall test phase)，rats received ten additional extinction trials in context A。Experiment 2：On Day 1，rats were transported to context A and context B to habituate them to the context； On Day 2(fear conditioning phase)，rats received five pairings of tone and footshock in context A；The next day(extinction training phase) ，rats received ten tone-alone