水稻钾离子通道基因oskat11的克隆表达载体的构建及其电生理.pdf

基因组学与应用生物学，2011 年，第30 卷，第4 期，第346-351 页 Genomics and Applied Biology, 2011, Vol.30, No.4, 346-351 研究报告 A Letter 水稻钾离子通道基因OsKAT1.1 的克隆、表达载体的构建及其电生理功能 1 2 1* 2 李俊林 高南 刘春生 苏彦华 1 山东农业大学资源与环境学院, 泰安, 271018; 2 土壤与农业可持续发展国家重点实验室, 中国科学院南京土壤研究所, 南京, 210008 * 通讯作者, csliu@ 摘 要 K+ 通道是植物高效吸收和体内运输K+ 的载体，对保证植物的钾素营养和增强抗逆性具有重要意 义。水稻生长在淹水环境中，其K+ 通道在长期适应物种立地条件的进化过程中可能形成了有别于拟南芥等 模式植物的独特功能特征和作用机制。本研究克隆了一个水稻KAT 型K+ 通道基因OsKAT1.1，并利用电生 理技术探讨其电生理功能。研究结果表明，通过一系列亚克隆过程，我们成功构建了适用于双电极电压钳和 膜片钳电生理研究的表达载体pCI-OsKAT1.1 和pTracer-CMV3-OsKAT1.1。进一步的电生理试验结果验证 了构建过程的准确性，并表明水稻OsKAT1.1 是一个由膜电位控制的吸收型K+ 通道。 关键词 水稻钾通道OsKAT1.1, 克隆, 表达载体构建, 电生理功能 Cloning, Construction of Expression Vectors and Electrophysiological Func- tion of Potassium Channel Gene OsKAT1.1 in Rice Li Junlin 1 Gao Nan 2 Liu Chunsheng 1* Su Yanhua 2 1 College of Resources and Environment of Shandong Agricultural University, Taian, 271018; 2 State Key Laboratory of Soil and Sustainable Agricul- ture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008 * Corresponding author, csliu@ DOI: 10.3969/gab.030.000346 Abstract As one of major mechanisms mediating K+ acquisition and redistribution in plants, K+ channels are key machineries in supporting potassium nutrition as well as improving stress resistance. Growing in flooding paddy soil, rice K+ channels could form preferential functional characteristics and regulatory mechanisms adaptive to the circumstances that are different from their counterparts found in dry land model plants such as Arabidopsis thaliana. However, our knowledge on rice K+ channels is not well documented. Therefore, in this report, a KAT-type rice K+ channel gene OsKAT1.1 was cloned and functionally studied through electrophysiological ap- proaches. Expression vectors pCI-OsKAT1.1 and pTracer-CMV3-OsKAT1.1 were successfully constructed for downstream studies with two-ele