响应面分析法优化黄精多糖提取工艺参数.pdfVIP

198 2007, Vol. 28, No. 07 食品科学 ※工艺技术 响应面分析法优化黄精多糖提取工艺参数 1 2 3 4 陈钢，陈红兰，苏伟，张桂芝 ( 1.南昌大学生命科学学院，食品科学教育部重点实验室，江西南昌 　330047 ； 2.江西应用技术职业学院，江西赣州 　34 1000 ；3 .江西科技师范学院生命科学学院，江西南昌　 3300 13 ； 4 .新疆农业大学食品科学学院，新疆乌鲁木齐 　830052) 摘 要：在黄精多糖提取体系中，利用响应面分析法(response surface methodology)对在单因素试验基础上选取的料 液比、提取温度和提取时间三个主要因素，以黄精多糖提取率为响应值，对其提取工艺参数进行优化，得出黄精 多糖水提取的最佳工艺条件为：料液比是2 1.5 : 1 ，提取温度：7 3 .5 ℃，提取时间：2 .5h ，黄精多糖的实际提取率 可达12 .2 5% ，比单因素试验最高提取率高出10 .6% 。 关键词：黄精多糖；提取；响应面分析 Optimization of Extraction Technique of Poly onatic Sibiricum Polysaccharides by Response Surface Analysis 1 2 3 4 CHEN Gang ，CHEN Hong-lan ，SU Wei ，ZHANG Gu i-zhi (1.College of Life Science, Nanchang University, Key Laboratory of Food Science, Ministry of Education, Nanchang 330047, China ；2.Jiangxi College of Applied Technology, Ganzhou 34 1000, China ； 3.College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 3300 13, China ； 4.College of Food Science, Xinj iang Agricultural Univercity, Urumqi 830052, China) Abstract ：In this paper, aiming at optimization of the extraction of Poly onatic Sibiricum polysaccharides (PSP), test factors and levels were firstly selected by one-factor tests. On the basis of previous results and according to the central composite test design principles, the method of response surface method (RSM) with 3 factors and 3 levels was adopted. Response surface and contour are finally graphed with the extraction rate of PSP as the response value. The results showed that the optimum conditions of PSP extraction are: ratio of