辛伐他汀对心肌梗死后心肌骨桥蛋白表达的影响及心室功能的相关性.docVIP

辛伐他汀对心肌梗死后心肌骨桥蛋白表达的影响及心室功能的相关性 蒋正英1，覃 数2 （400030 重庆，重庆市肿瘤研究所重症医学科1；400016 重庆，重庆医科大学附属第一医院心血管内科2重庆市） [摘要] 目的 在大鼠心肌梗死模型上，观察辛伐他汀是否下调OPNmRNA及蛋白表达，及其与改善大鼠心肌梗死后心室重塑与心脏功能的关系。方法 制备MI模型，予辛伐他汀干预。分为三组：Sim组，MI组及假手术组。4周后分别测定左、右心室质量指数，血流动力学指标，HE染色观察心肌组织病理学改变，运用免疫组化、RT-PCR及Western bolt方法检测OPN蛋白及mRNA表达并与假手术组比较。结果 MI组较Sham组左、右室质量指数均有所增加（P0.01）,血流动力学恶化（P0.01），心肌中OPNmRNA及蛋白表达量明显增加（P0.01）；Sim组较MI组左、右室质量指数均有下降（P0.01）,血流动力学改善（P0.01），心肌中OPNmRNA及蛋白表达量明显降低（P0.01）；MI组OPN mRNA表达与左、右心室重量指数呈显著正相关（r=0.760，P=0.029；r=0.804，P=0.016），与LVEF、FS变化呈显著负相关（r=-0.791，P=0.020；r=-0.841，P=0.009）。结论 辛伐他汀改善MI后左室重塑和心功能，这可能与其在基因转录和蛋白质翻译水平抑制OPN表达等综合因素有关。 [关键词] 辛伐他汀；骨桥蛋白；心肌梗死；心肌重塑 [中图法分类号] R542.23 [文献标志码] A The Effect of Simvastatin on Osteopontin Expression and it’s Relationship With Ventricular Remodeling After Myocardlal Infarction Jiang Zhengying1，Qin Shu2 (1 Department of Intensive Care Medicine. The Cancer Hospital of Chongqing, Chongqing, 400030，China;2Department of Cardiology．the First Affiliated Hospital，Chongqing University of Medical Sciences，Chongqing, 400016，China Chongqing, 400021) [Abstract] Objective To investigate the effect of simvastatin on the abatement of the mRNA,protein expression of oesteopontin(OPN) and the improvement of the cardiac remodeling and cardiac disfunction after the myocardial infarction in rats. Method MI models was maded in Wistar rats . There were three groups: Sim group, MI group , Sham group. Afer 4weeks, ventricular weight index and hemodynamics were observed, mRNA and protein expression of OPN in cardiac muscles were measured by immunohistochemical method, RT-PCR and Western bolt . Results Compared with the sham group,the ventricular weight index and the mRNA and protein expression were increased in rats with myocardiac infarctionthere （P0.01）and the hemodynamics was worsened（P0.01）while compared with the group with simvastatin intervention , the cardiac weight index and hemodynamics were improved and the mRNA and protein expression of OPN wer