a sphingosine kinase form 2 knockout sensitizes mouse myocardium to ischemiareoxygenation injury and diminishes responsiveness to ischemic preconditioning一种鞘氨醇激酶2淘汰赛糖分会让老鼠心肌缺血性预处理ischemiareoxygenation损伤和减少响应能力.pdfVIP

Hindawi Publishing Corporation Oxidative Medicine and Cellular Longevity Volume 2011, Article ID 961059, 8 pages doi:10.1155/2011/961059 Research Article A Sphingosine Kinase Form 2 Knockout Sensitizes Mouse Myocardium to Ischemia/Reoxygenation Injury and Diminishes Responsiveness to Ischemic Preconditioning Donald A. Vessey,1, 2 Luyi Li,1 Zhu-Qiu Jin,3 Michael Kelley,1 Norman Honbo,4 Jianqing Zhang,2 and Joel S. Karliner2, 4, 5 1 Liver Study Unit, Veterans Aff airs Medical Center, San Francisco, CA 94121, USA 2 Department of Medicine, University of California, San Francisco, CA 94143, USA 3 Department of Pharmaceutical Science, South Dakota State University, Brookings, SD 57007, USA 4 Cardiology Section, Veterans Aff airs Medical Center, San Francisco, CA 94121, USA 5 Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA Correspondence should be addressed to Donald A. Vessey, donald.vessey@ Received 9 December 2010; Accepted 10 January 2011 Academic Editor: Kenneth Maiese Copyright © 2011 Donald A. Vessey et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Sphingosine kinase (SphK) exhibits two isoforms, SphK1 and SphK2. Both forms catalyze the synthesis of sphingosine 1-phosphate (S1P), a sphingolipid involved in ischemic preconditioning (IPC). Since the ratio of SphK1 : SphK2 changes dramatically with aging, it is important to assess the role of SphK2 in IR injury and IPC. Langendorff mouse hearts were subjected to IR (30 min equilibration, 50 min global ischemia, and 40 min reperfusion). IPC consisted of 2 min of ischemia and 2 min of reperfus