acanthus ebracteatus vahl. ethanol extract enhancement of the efficacy of the collagen scaffold in wound closure a study in a full-thickness-wound mouse model爵床属ebracteatus vahl。.pdfVIP

Hindawi Publishing Corporation Journal of Biomedicine and Biotechnology Volume 2012, Article ID 754527, 8 pages doi:10.1155/2012/754527 Research Article Acanthus ebracteatus Vahl. Ethanol Extract Enhancement of the Efficacy of the Collagen Scaffold in Wound Closure: A Study in a Full-Thickness-Wound Mouse Model Jutamas Somchaichana,1 Tanom Bunaprasert,2 and Suthiluk Patumraj1, 3 1 Inter-Department of Physiology, Graduate School and Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand 2 Laboratory of Tissue Engineering and Department of Otolaryngology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand 3 Center of excellence for Microcirculation, Department of Physiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand Correspondence should be addressed to Suthiluk Patumraj, suthilukp@ Received 4 July 2012; Revised 25 August 2012; Accepted 26 August 2012 Academic Editor: Rudi Beyaert Copyright © 2012 Jutamas Somchaichana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Acanthus ebracteatus Vahl. is a Thai herb that is effective in wound healing. We sought to quantitatively determine whether or not the combined application of Acanthus ebracteatus Vahl. and a collagen scaffold will increase wound closure and angiogenesis. Balb/c mice (body weight: 22–25 g) were anesthetized with sodium thiopental. The dorsal skin incision measuring 1.5 × 1.5 cm was made and then deepened using scissors to produce a full-thickness incision down to the level of the panniculus carnosus. The size of the wound was approximately 10% of the total body sur