antioxidant and preventive effects of extract from nymphaea candida flower on in vitro immunological liver injury of rat primary hepatocyte cultures抗氧化剂和预防的影响从睡莲属念珠菌花提取物在体外免疫肝损伤大鼠原代肝细胞培养.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 497673, 8 pages doi:10.1093/ecam/nep003 Original Article Antioxidant and Preventive Effects of Extract from Nymphaea candida Flower on In Vitro Immunological Liver Injury of Rat Primary Hepatocyte Cultures Jun Zhao,1, 2 Tao Liu,3 Long Ma,3 Ming Yan,2 Zhengyi Gu,2 Yi Huang,2 Fang Xu,2 and Yu Zhao1 1 Department of Traditional Chinese Medicine and Natural Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China 2 Xinjiang Key Laboratory for Research and Development of Uighur Medicine, Institute of Materia Medica of Xinjiang, Urumqi, 830002, China 3 College of Public Health, Xinjiang Medical University, Urumqi, 830054, China Correspondence should be addressed to Jun Zhao, zhaojun21cn@163.com Received 6 September 2008; Accepted 12 January 2009 Copyright © 2011 Jun Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Nymphaea candida is traditional Uighur medicine that is commonly used to treat head pains, cough, hepatitis and hypertension in Xinjiang of China. In this article, the extract of N. candida was measured for antioxidant activity, using 1,1-diphenyl-2- picrylhydrazyl (DPPH) radicals scavenging assay and reducing power determination, and compared with those of the positive controls of butylated hydroxytoluene (BHT) and gallic acid (GA). The active extract was further purified by liquid-liquid partition to afford four fractions, of which the ethyl acetate-soluble (EA) fraction (NCE) exhibited the strongest antioxida