antioxidant potential of momordica charantia in ammonium chloride-induced hyperammonemic rats苦瓜的抗氧化潜力铵chloride-induced hyperammonemic老鼠.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 612023, 7 pages doi:10.1093/ecam/nep227 Original Article Antioxidant Potential of Momordica Charantia in Ammonium Chloride-Induced Hyperammonemic Rats A. Justin Thenmozhi and P. Subramanian Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamil Nadu, India Correspondence should be addressed to P. Subramanian, psub@rediff Received 26 June 2009; Accepted 1 December 2009 Copyright © 2011 A. J. Thenmozhi and P. Subramanian. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The present study was aimed to investigate the antioxidant potential of Momordica charantia fruit extract (MCE) in ammonium chloride-induced (AC) hyperammonemic rats. Experimental hyperammonemia was induced in adult male Wistar rats (180–200 g) by intraperitoneal injections of ammonium chloride (100 mg kg−1 body weight) thrice a week. The effect of oral administration (thrice a week for 8 consecutive weeks) of MCE (300 mg kg−1 body weight) on blood ammonia, plasma urea, serum liver marker enzymes and oxidative stress biomarkers in normal and experimental animals was analyzed. Hyperammonemic rats showed a significant increase in the activities of thiobarbituric acid reactive substances, hydroperoxides and liver markers (alanine transaminase, aspartate transaminase and alkaline phosphatase), and the levels of glutathione peroxidase, superoxide dismutase, catalase and reduced glutathione were decreased in the liver and brain