application of metabolomics in traditional chinese medicine differentiation of deficiency and excess syndromes in patients with diabetes mellitus代谢组学在中药中的应用差异化不足和过剩症状的糖尿病患者.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2012, Article ID 968083, 11 pages doi:10.1155/2012/968083 Research Article Application of Metabolomics in Traditional Chinese Medicine Differentiation of Deficiency and Excess Syndromes in Patients with Diabetes Mellitus Tao Wu,1, 2 Ming Yang,3 Hua-Feng Wei,4 Song-Hua He,4 Shun-Chun Wang,5 and Guang Ji2 1 Center of Chinese Medicine Therapy and Systems Biology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China 2 Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China 3 Department of Medicament, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China 4 Department of Internal Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China 5 Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China Correspondence should be addressed to Shun-Chun Wang, wsc@ and Guang Ji, jiliver@ Received 18 February 2012; Revised 9 April 2012; Accepted 16 April 2012 Academic Editor: Wei Jia Copyright © 2012 Tao Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Metabolic profiling is widely used as a probe in diagnosing diseases. In this study, the metabolic profiling of urinary carbohydrates was investigated using gas chromatography/mass spectrometry (GC/MS) and multivariate statistical analysis. The kernel-based orthogonal projections to latent structures (K-OPLS) model were established and validated to distingui