aqueous extract of black maca (lepidium meyenii) on memory impairment induced by ovariectomy in mice水提物的黑玛咖(lepidium meyenii)引起的记忆障碍小鼠卵巢切除术.pdfVIP

Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2011, Article ID 253958, 7 pages doi:10.1093/ecam/nen063 Original Article Aqueous Extract of Black Maca (Lepidium meyenii ) on Memory Impairment Induced by Ovariectomy in Mice Julio Rubio,1, 2 Wang Qiong,1 Xinmin Liu,1 Zhen Jiang,1 Haixia Dang,1 Shi-Lin Chen,1 and Gustavo F. Gonzales2 1 Research Center for Pharmacology and Toxicology, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China 2 Department of Biological and Physiological Sciences, Faculty of Sciences and Philosophy and Instituto de Investigaciones de la Altura, Universidad Peruana Cayetano Heredia, PO Box 1843, Lima, Peru Correspondence should be addressed to Xinmin Liu, liuxinminuae@ Received 30 May 2008; Accepted 11 September 2008 Copyright © 2011 Julio Rubio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The present study aims to test two different doses of aqueous extract of black maca on learning and memory in ovariectomized (OVX) mice and their relation with malonalehyde (MDA), acetylcholinesterase (Ache) and monoamine oxidase (MAO) brain levels. Female mice were divided into five groups: (i) naive (control), (ii) sham, (iii) OVX mice and OVX mice treated with (iv) 0.50 g kg−1 and (v) 2.00 g kg−1 black maca. Mice were orally treated with distilled water or black maca during 35 days starting 7 days after surgery. Memory and learning were assessed using the water Morris maze (from day 23–27) and the step-down avoidance test (days 34 and 35). At the end of each treat