cardiac ppar protein expression is constant as alternate nuclear receptors and pgc-1 coordinately increase during the postnatal metabolic transition心脏ppar蛋白表达是常数替代核受体和pgc-1协调增加产后期间代谢转变.pdfVIP

Hindawi Publishing Corporation PPAR Research Volume 2008, Article ID 279531, 8 pages doi:10.1155/2008/279531 Research Article Cardiac PPARα Protein Expression is Constant as Alternate Nuclear Receptors and PGC-1 Coordinately Increase During the Postnatal Metabolic Transition Norman E. Buroker, Xue-Han Ning, and Michael Portman Department of Cardiology, Children’s Hospital and Regional Medical Center, 4800 Sand Point Way N.E., Seattle, WA 98105, USA Correspondence should be addressed to Michael Portman, michael.portman@ Received 20 June 2007; Accepted 22 July 2007 Recommended by Brian N. Finck Gene expression data obtained in mouse heart indicate that increased expression for the nuclear receptor, peroxisomal prolif- erator activated receptor α (PPARα), prompts the postnatal transition from predominantly carbohydrate to fatty acid oxidation preference. However, no phenotypic or proteomic data are available to confirm downstream signaling and metabolic transition in mice. We studied the hypothesis that shifts in nuclear receptor expression trigger the newborn metabolic switch in a newborn sheep. This species is well characterized with regards to developmental changes in substrate oxidative metabolism. Heart tissues from fetal (130 days gestation), newborn ≤ 24 hours, and 30-day old lambs were evaluated for protein expression from multiple enzymes controlling oxidative metabolism as well as principal nuclear receptors and coactivators. Although muscle and liver type carnitine palmitoyl transferases I showed no significant changes to correspond to the metabolic transition, hexokinase II protein content showed a profound transient drop, and pyruvate dehydrogenase kinase steadily increased. PPARα showed no increases preceding or during the transition, while peroxisomal proliferator activat