remediation of pb-resistant bacteria to pb polluted soilpb-resistant细菌修复铅污染土壤.pdf

Journal of Environmental Protection, 2011, 2, 130-141 doi:10.4236/jep.2011.22015 Published Online April 2011 (http://www.SciRP.org/journal/jep) Remediation of Pb-Resistant Bacteria to Pb Polluted Soil 1 1 1 1 2 1 Bao Chen , Jia-nan Liu , Zheng Wang , Lei Dong , Jing-hua Fan , Juan-juan Qu 1Resource and Environmental College, Northeast Agricultural University, Harbin, China; 2University of Florida, Institute of Food and Agricultural Sciences, Indian River Research and Education Center, Gainesville, USA. Email: {Chenbao139, southliu, dongjiadexiaolei}@163.com, soszheng00@, creamfan@, juanjuanqu@126.com Received November 4th, 2010; revised December 17th, 2010; February 10th, 2011. ABSTRACT To show the remediation of Pb-resistant bacteria to Pb polluted soil, several indices including microbial counts, soil enzyme activity, microbial community diversity and soil Pb concentration were investigated. Two Pb-resistant bacteria were filtrated and identified by previous study as Bacillus pumilus and Pseudomonas aeruginosa (GeneBank Accession No. FJ402988 and GU017676) and inoculated to soil planted with cabbages. Soil with different Pb application rates were incubated for a period of 0, 12, 24, 36, 48 days in greenhouse. Results indicated the count of bacteria in 1000 mg/kg Pb treated soil greatly affected by inoculating Pb-resistant bacteria, which was raised about 237% and 347% compared with control. Soil urease and invertase were intensified 37.9% and 65.6% after inoculation compared with control. Phosphatase activity was inhibited by inoculation of Bacillus pumilus. Catalase activity was intensified about 64.2% in 24 days incubation but decrease in the following days. Microbial community diversity analyzed by po- lymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) also proved that the samples in