repeated exposure to cruciferous allyl nitrile protects against chemically induced skin inflammation in the mouse反复接触十字花科烯丙腈防止化学诱导小鼠皮肤炎症.pdf

Food and Nutrition Sciences, 2012, 3, 1037-1042 1037 /10.4236/fns.2012.38137 Published Online August 2012 (http://www.SciRP.org/journal/fns) Repeated Exposure to Cruciferous Allyl Nitrile Protects against Chemically Induced Skin Inflammation in the Mouse * Hideji Tanii , Tomomi Higashi, Masashi Demura, Kiyofumi Saijoh Department of Hygiene, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan. * Email: taniih@med.kanazawa-u.ac.jp Received May 17th, 2012; revised June 12th, 2012; accepted June 19th, 2012 ABSTRACT Repeated exposure to cruciferous allyl nitrile can induce antioxidant and phase 2 detoxification enzymes in various tis- sues. In the present study, we examined the effect of five days repeated exposure to allyl nitrile at subtoxic levels (0 - 400 µmol/kg/day) on the mouse ear. There was an increase in catalase activity in the ear at 100 - 400 µmol/kg/day, while elevated quinone reductase activity was observed at 400 µmol/kg/day only. Next, after repeated allyl nitrile ex- posure (0 - 400 µmol/kg/day), the skin irritant croton oil was applied to the ear to induce skin acute inflammation (oe- dema). Compared with the 0 µmol/kg/day group, animals in the 100 and 400 µmol/kg/day pre-treatment groups showed reduced oedematous response to croton oil. The reduced oedematous response was inversely associated with enhanced myeloperoxidase activity used as index of the presence of neutrophils. These data suggest that repeated exposure to allyl nitrile at subtoxic levels contributes to protection against croton oil-induced ear dermatitis, potentially through decreasing reactive oxygen species and through infiltration of neutrophils. Keywords: Allyl Nitrile; Croton Oil; Catalase; Quinone Reductase; Skin; Inflammation 1. Introduction