research on influences of transient high iop during lasik on retinal functions and ultrastructure研究瞬态高眼压的影响在lasik手术视网膜功能和超微结构.pdf

Hindawi Publishing Corporation Journal of Ophthalmology Volume 2009, Article ID 230528, 5 pages doi:10.1155/2009/230528 Research Article Research on Influences of Transient High IOP during LASIK on Retinal Functions and Ultrastructure Haixia Zhao, Yude Ai, Chunmei Niu, Wenying Guan, Xiaoling Li, and Liru Qin Department of Ophthalmology, Affi liated Hospital of Inner Mongolia Medical College, Hohhot 010050, China Correspondence should be addressed to Haixia Zhao, zhaohaixia@ Received 15 September 2009; Accepted 10 December 2009 Recommended by Edward Manche Objectives. To study the influences of transient high intraocular pressure(IOP) during LASIK on retinal functions and ultrastructure. Methods. Thirty-two New Zealand white rabbits were randomly divided into normal control, experimental control, negative suction 20 s and negative suction 3 min groups. The experimental control group was treated only by laser. Rabbit eyes received suction for different periods of time (20 s, 3 min) by negative pressure generator in different groups. The changes of neuro-optic and retinal ultrastructure were observed under electron and light microscopes; retinal neurofunctional changes were observed with flash-visual evoked potential (F-VEP) and flash-electroreinogram (F-ERG). Results. There was no obvious change in optic nerve, retina, ERG a-wave and b-wave in normal control and experimental control groups. There were slight changes in tissues of optic nerve and retina at various times of suction 20 s compared with control group, and a sharp change in suction 3 min group within 14d after operation, but these changes recovered at 28d . Amplitude of ERG b-wave observed at different time will decrease with suction periods prolonged. It can recover to normal level with the