scfv antibody principles and clinical applicationscfv抗体原理和临床应用.pdf

Hindawi Publishing Corporation Clinical and Developmental Immunology Volume 2012, Article ID 980250, 15 pages doi:10.1155/2012/980250 Review Article scFv Antibody: Principles and Clinical Application Zuhaida Asra Ahmad,1 Swee Keong Yeap,2 Abdul Manaf Ali,3 Wan Yong Ho,1 Noorjahan Banu Mohamed Alitheen,1 and Muhajir Hamid1, 4 1 Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia 2 Institute of Bioscience, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia 3 Faculty of Agriculture and Biotechnology, Universiti Sultan Zainal Abidin, Kampus Kota, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Malaysia 4 Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia Correspondence should be addressed to Noorjahan Banu Mohamed Alitheen, noorjahan@.my Received 10 November 2011; Accepted 7 January 2012 Academic Editor: T. Nakayama Copyright © 2012 Zuhaida Asra Ahmad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments. Basically, scFv antibodies produced from phage display can be genetically fused to the marker proteins, such as fluorescen