smart integrated sensor for multiple detections of glucose and l-lactate using on-chip electrochemical system智能集成传感器的多个检测葡萄糖和l-lactate使用芯片上的电化学系统.pdf

Hindawi Publishing Corporation Journal of Sensors Volume 2011, Article ID 190284, 7 pages doi:10.1155/2011/190284 Research Article Smart Integrated Sensor for Multiple Detections of Glucose and L-Lactate Using On-Chip Electrochemical System Tomoyuki Yamazaki,1, 2 Takaaki Ikeda,1 Byounghyun Lim,1 Koichi Okumura,1 Makoto Ishida,1, 3 and Kazuaki Sawada1, 3 1 Department of Electronic and Information Engineering, Toyohashi University of Technology, C3-601, 1-1 Hibarigaoka, Tenpaku, Toyohashi, Aichi, 441-8580, Japan 2 New Product Development Department, Hioki E. E. Corporation, 81 Koizumi, Ueda, Nagano 386-1192, Japan 3 JST-CREST, Sanban-cho Building, 4F, 5 San ban-cho, Chiyoda-ku, Tokyo 102-0075, Japan Correspondence should be addressed to Tomoyuki Yamazaki, yamazaki-t@int.ee.tut.ac.jp Received 14 February 2011; Accepted 26 April 2011 Academic Editor: Michele Penza Copyright © 2011 Tomoyuki Yamazaki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Multiple sensor electrodes, a supplementary electrode, a reference electrode, and signal-processing circuits were integrated on a single chip to develop a chip-shaped electrochemical sensing system. L-lactate and glucose were measured using on-chip working electrodes modified by polyion complex to immobilize lactate oxidase and glucose oxidase, respectively. Cyclic voltammetry measurements were conducted using an on-chip potentiostat. Selective and quantitative detection of glucose and L-lactate and the interference behavior were studied. Hydrogen peroxide generated by enzymatic reactions was detected by an increase in anodic