spt5 affects the rate of mrna degradation and physically interacts with ccr4 but does not control mrna deadenylationspt5影响信使rna降解率和身体与mrna deadenylation ccr4但不控制.pdf

American Journal of Molecular Biology, 2012, 2, 11-20 AJMB /10.4236/ajmb.2012.21002 Published Online January 2012 (http://www.SciRP.org/journal/ajmb/) SPT5 affects the rate of mRNA degradation and physically interacts with CCR4 but does not control mRNA * deadenylation 1 1 2 Yajun Cui , Yueh-Chin Chiang , Palaniswamy Viswanathan, Darren J. Lee, Clyde L. Denis Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, USA Email: cldenis@ Received 12 August 2011; revised 16 September 2011; accepted 25 September 2011 ABSTRACT in the cytoplasm. Regulation of the rate of mRNA decay is therefore an important control point in determining the The CCR4-NOT complex has been shown to have mul- abundance of cellular mRNA. A principal mRNA decay tiple roles in mRNA metabolism, including that of pathway in eukaryotic cells is initiated by shortening of transcriptional elongation, mRNA transport, and nu- the mRNA poly(A) tail at the 3′ end (deadenylation), clear exosome function, but the primary function of followed by removal of the 5′ cap structure (decapping) CCR4 and CAF1 is in the deadenylation and degra- and subsequent 5′ to 3′ exon