大孔树脂纯化川芪眼用凝胶水提液工艺研究-中国中医药信息杂志.PDF

2015 年12月第22卷第12期 中国中医药信息杂志 ·79· 大孔树脂纯化川芪眼用凝胶水提液工艺研究 于娟,冯伟红,杜茂波,沈硕,刘淑芝 中国中医科学院中药研究所,北京 100700 摘要：目的 研究大孔吸附树脂富集川芪眼用凝胶水提液中有效成分的工艺条件及参数。方法 以黄芪甲 苷和阿魏酸为指标成分,采用高效液相色谱法进行含量测定,优选大孔树脂纯化的最佳工艺。结果 D101 大孔 吸附树脂对待测成分的纯化效果较好,最佳精制工艺条件为：上样浓度为 0.3 g 原药材/mL,上样量为 6 倍上样 液,洗脱液为 6 倍树脂量的 70%乙醇溶液,动态洗脱流速为 6 BV/h。结论 D101 大孔吸附树脂纯化川芪眼用凝 胶水提液的最佳工艺稳定、可行。 关键词：大孔吸附树脂；黄芪甲苷；阿魏酸；纯化 DOI：10.3969/j.issn.1005-5304.2015.12.020 中图分类号：R283.5 文献标识码：A 文章编号：1005-5304(2015)12-0079-05 Study on Extraction Technology of Chuanqi Ophthalmic Gel Purified by Macroporous Resin YU Juan, FENG Wei-hong, DU Mao-bo, SHEN Shuo, LIU Shu-zhi (Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China) Abstract ：Objective To investigate the technological conditions and parameters of effective components in water extraction of Chuanqi Ophthalmic Gel purified with macroporous resin. Methods The contents of astragaloside and ferulic acid were set as indexes and determined by HPLC, with a purpose to evaluate the optimal macroporous resin purification process. Results The optimal macroporous resin is D101. The optimal purification technology is as follows：The sample concentration is 0.3 g crude drug/mL；the amount of resin-like fluid is 6 BV；dynamic adsorption flow rate is 6 BV/h；concentration eluenta is 70% ethanol；the amount of eluenta fluid is 6 BV. Conclusion The technology of D101 macroporous resin for purification of water extraction Chuanqi Ophthalmic Gel is stable and feasible. Key words ：macroporous resin；astragaloside；ferulic acid；purification 益气活血类中药对眼底疾病具有良好疗效,而黄 Sartorius 公司),恒温水浴锅(上海医疗器械五厂), 芪、川芎是最常用的药味。以黄芪、川芎组方的川芪 电子天平(常熟市双杰测试仪器厂)。 眼用凝胶为模型药进行水提或醇提工