植物miR408家族进化与分子特性分析-应用与环境生物学报.DOC

植物miR家族进化分析(刘炜婳1 倪珊珊1林争春1 玉玲1 赖钟雄1(( 1福建农林大学园艺植物生物工程研究所 福州 350002 摘 要为了解植物miR408家族成员的进化规律及分子特性，对34个物种的49个408前体序列和64个miR408成熟体序列进行进化规律分析对植物miR408家族成员的二级结构和靶基因进行预测分析对成熟体序列进行比对分析。结果表明：miR408家族成员分布在34个植物物种中，分布较为广泛，苔藓类植物很可能是miR408家族进化的祖先进化分析表明，物种特异性是影响408前体进化特性的重要因素，而序列保守性是影响成熟体进化特性的主要因素序列分析表明，由5p臂上形成的miR408成熟体的序列特异性较大，由3p臂上形成的miR408成熟体的序列保守较高二级结构分析发现植物miR408家族具有典型的茎环二级结构，茎序列的保守性高于环序列，3p臂上茎序列的保守性高于5p臂上茎序列靶基因预测分析。关键词植物；miR408家族进化规律分子靶基因预测 Analysis of evolution and molecular characteristics of miR408 family in plants( LIU Weihua1, NI Shanshan1, LIN Zhengchun1, LIN Yuling1 LAI Zhongxiong1** 1Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou 350002, China Abstract Objectives: To explore the evolution rules and molecular characteristics of the miR408 family members. Methods: The evolution analysis of 49 MIR408 and 64 miR408 sequences in 34 species was conducted, the secondary structures and target genes of the miR408 family were predicted, and the differences of mature miR408 family were compared in plants. Results: The results showed that the miR408 family members were distributed to 34 species of plants, which suggested that the bryophytes might be the evolutionary ancestors of the miR408 family. The evolution analysis indicated that the specificity of species was an important factor affecting the precursor evolution of MIR408 family, while the conservation of sequences was one of the main factors affecting the evolution of the mature miR408 family. The sequence analysis indicated that the members of miR408-5p were quite specific, on the contrary, conserved for the members of miR408-3p. Mfold predicted that pre-miR408 spontaneously forms a typical, stable stem-loop secondary structure and the stem sequence was more conserved than loop sequence, and the miR408-3p sequence was more conserved than the miR408-5p sequence. Target gene analysis showed that miR408 family members mainly regulated plant growth, development and respon