流形切割及有限元网格覆盖下的三维流形单元生成-岩石力学与工程学报.PDF

遗 传 学 报 Acta Genetica Sinica , October 2006, 33 (10)：881–891 ISSN 0379-4172 Microarray Analysis of Gene-Expression Profile in Hepatocel- lular Carcinoma Cell, BEL-7402, with Stable Suppression of hLRH-1 via a DNA Vector-based RNA Interference 1,① 1 1 1 WANG Shui-Liang , LAN Feng-Hua , ZHUANG Yue-Peng , LI Hui-Zhong , HUANG Li- 1 1 1 1 1 2 ang-Hu , ZHENG De-Zhu , ZENG Jian , DONG Li-Hong , ZHU Zhong-Yong , FU Ji-Liang 1. Center for Molecular Medicine, Institute of Laboratory Medicine, Fuzhou General Hospital, Fuzhou 350025, China; 2. Department of Medicine and Life Science, Tongji University, Shanghai 200092, China Abstract: To establish a cell line with a permanent suppression of hLRH-1 in this study, a stable RNAi vector (pSineohLRH-1) targeting hLRH-1 was constructed and introduced into hepatocellular carcinoma cell, BEL-7402. By semiquantitative RT-PCR analysis, the expression of hLRH-1 in BEL-7402 cells carrying pSineohLRH-1 was shown to be significantly suppressed by up to ~60%. In addition, microarray analysis was carried out to assess the extent of altered gene expression in BEL-7402 cells with stable knockdown of hLRH-1. Direct comparison of gene-expression profiles of more than 18 000 genes showed that 405 of the expressed genes in hLRH-1-knockdown cells differed dramatically in expression levels from those in controls, which suggested the even ex- tensive biological functions of hLRH-1. Interestingly, among those differentially expressed genes, some are cancer-associated such as Gadd45β and PTEN, and their expressions were further validated. Althou