内生菌对茅苍术生长和挥发油积累影响的研究.pdfVIP

ABSTRACT Abstract Atractylodes lancea (Thunb.) DC. is a kind of composite perennial herb, whose rhizome(atractylodes) is the native medicinal material in Jiangsu Province. Essential oil is the main active component of atractylodes, whose function is to deprive the evil wetness, to invigorate the spleen and to improve eyesight. Due to the exhaustion of resources of A. lancea and the difficulties of cultivation, the resources of A. lancea are rare. In order to protect and develop the resource of A. lancea , we attempt to study the effect of endophytic fungal on the growth and essential oil production of A. lancea , from the perspective of the relationship between microorganisms and plants On the first, the effect of different temperatures, different seed soaking time on the seed germination rate and growth were studied. Then the growth, physiology index and the content of essential oil were observed. The results showed that temperature(20 ℃) and seed soaking time(48h) were the best conditions for seed germination. AL3 (Sclerotium sp.), AL4 (Cunninghamella sp.), AL11 (not identified) and AL12 (Gilmaniella sp.) all improve the seed germination rate and potential. AL12 and AL3 increased the biomass by 34.2%(P0.01) and 46.9%(P0.01), increased the average number of roots of host by 76.0% (P0.01) and 65.8%(P0.01) respectively. AL4 and AL11 had no effects on growth of the hosts. The SOD and POD activity of AL11 and AL12 treatment increased slightly over the control. All of four species of endophytic fungi improved the content and changed the relative percentage of four important components in essential oil (atractylone, hinesol, β-eudesmol and atractylodin). Effects of endophytic fungi on g