原创力文档a low-cost microfluidic chip for rapid genotyping of malaria-transmitting mosquitoes一种低成本的微流控芯片快速基因分型的药蚊子.pdfVIP
- 1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
- 2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击 这里二次下载。
- 3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
- 4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
- 5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
- 6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
- 7、VIP文档为合作方或网友上传，每下载1次， 网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
a low-cost microfluidic chip for rapid genotyping of malaria-transmitting mosquitoes一种低成本的微流控芯片快速基因分型的药蚊子
A Low-Cost Microfluidic Chip for Rapid Genotyping of
Malaria-Transmitting Mosquitoes
1 1 1 2 2 1
Changchun Liu , Michael G. Mauk , Robert Hart , Mariangela Bonizzoni , Guiyun Yan , Haim H. Bau *
1 Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, Pennsylvania, United States of America, 2 College of Health
Sciences, University of California Irvine, Irvine, California, United States of America
Abstract
Background: Vector control is one of the most effective measures to prevent the transmission of malaria, a disease that
causes over 600,000 deaths annually. Around 30–40 Anopheles mosquito species are natural vectors of malaria parasites.
Some of these species cannot be morphologically distinguished, but have behavioral and ecological differences.
Emblematic of this is the Anopheles gambiae species complex. The correct identification of vector species is fundamental to
the development of control strategies and epidemiological studies of disease transmission.
Methodology/Principal Findings: An inexpensive, disposable, field-deployable, sample-to-answer, microfluidic chip was
designed, constructed, and tested for rapid molecular identification of Anopheles gambiae and Anopheles arabiensis. The
chip contains three isothermal amplification reactors. One test reactor operates with specific primers to amplify Anopheles
gambiae DNA, another with specific primers for Anopheles arabiensis DNA, and the third serves as a negative control. A
mosquito leg was crushed on an isolation membrane. Two discs, laden with mosquito tissue, were punched out of the
membrane and inserted into the two test chambers. The isolated, disc-bou
您可能关注的文档
- a cobb douglas stochastic frontier model on measuring domestic bank efficiency in malaysia柯布一道格拉斯随机前沿模型测量在马来西亚国内银行效率.pdf
- a coastal cline in sodium accumulation in arabidopsis thaliana is driven by natural variation of the sodium transporter athkt1;1沿海cline钠积累在拟南芥是由钠离子转运体的自然变异athkt1;1.pdf
- a collaborative filtering approach for protein-protein docking scoring functions蛋白质的协同过滤方法对接评分功能.pdf
- a collection of target mimics for comprehensive analysis of microrna function in arabidopsis thaliana一组目标模拟综合分析拟南芥的微rna的功能.pdf
- a combination of compositional index and genetic algorithm for predicting transmembrane helical segments的组合成分指数和遗传算法预测跨膜螺旋段.pdf
- a collaborative epidemiological investigation into the criminal fake artesunate trade in south east asia一个协作的流行病学调查罪犯假冒青蒿琥酯东南亚贸易.pdf
- a combination of independent transcriptional regulators shapes bacterial virulence gene expression during infection独立的转录监管机构的组合形状在感染的细菌毒力基因表达.pdf
- a combination of nutriments improves mitochondrial biogenesis and function in skeletal muscle of type 2 diabetic goto–kakizaki rats结合营养改善骨骼肌线粒体生物起源和函数2型糖尿病goto-kakizaki老鼠.pdf
- a combination of the kato-katz methods and elisa to improve the diagnosis of clonorchiasis in an endemic area, chinakato-katz的组合方法和elisa改善诊断支睾吸虫病的流行区,中国.pdf
- a combination of h2a.z and h4 acetylation recruits brd2 to chromatin during transcriptional activationh2a的组合。.pdf
- a major genetic locus in trypanosoma brucei is a determinant of host pathology锥虫属的主要遗传位点brucei行列式的主机病理学.pdf
- a machine learning approach for identifying amino acid signatures in the hiv env gene predictive of dementia机器学习方法对于识别氨基酸签名的艾滋病毒env基因预测痴呆.pdf
- a malaria vaccine that elicits in humans antibodies able to kill plasmodium falciparum一种疟疾疫苗,抒发人类抗体能够杀死恶性疟原虫.pdf
- a low dimensional description of globally coupled heterogeneous neural networks of excitatory and inhibitory neurons一个低维的描述全局耦合的异构兴奋和抑制性神经元的神经网络.pdf
- a malaria vaccine based on the polymorphic block 2 region of msp-1 that elicits a broad serotype-spanning immune response疟疾疫苗的基础上多态块2地区引发广泛的msp-1 serotype-spanning免疫反应.pdf
- a mammal-specific doublesex homolog associates with male sex chromatin and is required for male meiosis一个mammal-specific doublesex同族体同事与男性性染色质和需要男性减数分裂.pdf
- a many-body field theory approach to stochastic models in population biology一个多体的场理论方法在种群生物学随机模型.pdf
- a mammalian lost world in southwest europe during the late pliocene哺乳动物在上新世末期欧洲西南部的失落的世界.pdf
- a major ingredient of green tea rescues mice from lethal sepsis partly by inhibiting hmgb1绿茶的主要成分从致命的败血症部分通过抑制hmgb1救老鼠.pdf
- a man-made atp-binding protein evolved independent of nature causes abnormal growth in bacterial cells人造磷酸腺苷蛋白进化独立于自然导致细菌细胞异常生长.pdf
文档评论（0）