a map kinase dependent feedback mechanism controls rho1 gtpase and actin distribution in yeast一个map激酶依赖的反馈机制控制rho1 gtpase和肌动蛋白在酵母分布.pdfVIP

A MAP Kinase Dependent Feedback Mechanism Controls Rho1 GTPase and Actin Distribution in Yeast 1,3. 2. 1 1 1 3 Shuguang Guo , Xiaoyun Shen , Gonghong Yan , Dongzhu Ma , Xiaochun Bai , Suoping Li , Yu Jiang1* 1 Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States of America, 2 Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian, Liaoning, People’s Republic of China, 3 College of Life Science, Henan University, Kaifeng, People’s Republic of China Abstract In the yeast Saccharomyces cerevisiae the guanosine triphosphatase (GTPase) Rho1 controls actin polarization and cell wall expansion. When cells are exposed to various environmental stresses that perturb the cell wall, Rho1 activates Pkc1, a mammalian Protein Kinase C homologue, and Mpk1, a mitogen activated protein kinase (MAPK), resulting in actin depolarization and cell wall remodeling. In this study, we demonstrate a novel feedback loop in this Rho1-mediated Pkc1- MAPK pathway that involves regulation of Rom2, the guanine nucleotide exchange factor of Rho1, by Mpk1, the end kinase of the pathway. This previously unrecognized Mpk1-depedent feedback is a critical step in regulating Rho1 function. Activation of this feedback mechanism is responsible for redistribution of Rom2 and cell wall synthesis activity from the bud to cell periphery under stress conditions. It is also required for terminating Rho1 activity toward the Pkc1-MAPK pathway and for repolarizing actin cytoskeleton and restoring growth after the stressed cells become adapted. Citation: Guo S, Shen X, Yan G, Ma D, Bai X