外源性FHIT基因对奥曲肽诱导胃癌细胞MGC-803凋亡得影响.pdfVIP

(4) 转染FHIT 基因组细胞经奥曲肽处理后Bcl-2 蛋白表达减少，Bax 蛋 白表达增加。 结论：外源性FHIT 基因表达与奥曲肽可协同促进胃癌细胞MGC-803 凋亡， 其机制可能与BCL-2 家族中的凋亡相关蛋白改变有关。 关键词：脆性组氨酸三联体蛋白；奥曲肽；基因转染；胃肿瘤；细胞凋亡 4 Effects of fragile histidine triad gene on apoptosis of MGC-803 gastric cancer cell line induced by octreotide Abstract Objective: To obtain a MGC-803 cell line expressing FHIT protein by transfection ,and investigate the influence of exogenous FHIT gene expression on gastric cancer cells MGC-803 apoptosis induced by octreotide and the molecule mechanism. Methods: Human gastric cancer cells line MGC-803, in which there is expression loss of FHIT gene, was transfected with plasmid pRcCMV-FHIT using lipofectamine transfecting technique. After transfection, Western blot were used to analyse the expression of FHIT genes in positive cells screened out by G418. Each group cells was treated with octreotide at different concentrations. Cell proliferation assay were evaluated by MTT and apoptosis by flow cytometry, as well as the expression of Bcl-2 and Bax was detected with Western blot. Results: (1) Stable expression of FHIT protein in MGC-803 cells was established successful and the expression was detected in MGC-803 cells after transfection with FHIT gene. (2) There was concentration-dependent and time-dependent of octreotide on growth inhibition rate . (3)Results with octreotide treatment 48h later, the apoptotic rates of MGC-803 cells in FHIT gene group (26.777%±1.702%) was significantly higher than that in empty vector group (13.8%±0.511%) and untransfected group (12.634%±0.479%) (F =245.789，P0.05). (4) The expression of Bcl-2 protein was denclined while the expres