a novel high-throughput vaccinia virus neutralization assay and preexisting immunity in populations from different geographic regions in china一种新型高通量牛痘病毒中和试验和既存的免疫在中国人口来自不同的地理区域.pdfVIP

A Novel High-Throughput Vaccinia Virus Neutralization Assay and Preexisting Immunity in Populations from Different Geographic Regions in China 1,2 1 1 1 3 1 1 Qiang Liu , Weijin Huang , Jianhui Nie , Rong Zhu , Dongying Gao , Aijing Song , Shufang Meng , 2 1 Xuemei Xu , Youchun Wang * 1 Department of Cell Biology, National Institutes for Food and Drug Control, Key Laboratory of the Ministry of Health for Research on Quality and Standardization of Biotech Products, Beijing, China, 2 Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, China, 3 Beijing Blood Center, Beijing, China Abstract Background: Pre-existing immunity to Vaccinia Tian Tan virus (VTT) resulting from a large vaccination campaign against smallpox prior to the early 1980s in China, has been a major issue for application of VTT-vector based vaccines. It is essential to establish a sensitive and high-throughput neutralization assay to understand the epidemiology of Vaccinia-specific immunity in current populations in China. Methodology/Principal Findings: A new anti-Vaccinia virus (VACV) neutralization assay that used the attenuated replication-competent VTT carrying the firefly luciferase gene of Photinus pyralis (rTV-Fluc) was established and standardized for critical parameters that included the choice of cell line, viral infection dose, and the infection time. The current study evaluated the maintenance of virus-specific immunity after smallpox vac