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β-磷酸三钙煅烧骨修复兔股骨远端骨缺损-中国组织工程研究
Active Ingredient Content of Nanwuweizi and Beiwuweizi in Different Seasons by HPLC-DAD
J Int Transl Med, 2015, 3(4): 258-262; doi: 10.11910/2227-6394.2015.03.04.06
Research Article Open Access
Comparison of Active Ingredient Content
of Nanwuweizi and Beiwuweizi Collected in
Different Seasons Detected by HPLC-DAD
1 2*
YAO Feng , CHEN Da-shuai
1. Pharmacy of TCM, Nanyang Second General Hospital, Nanyang, Henan, 473012, China;
2. First Ward of Pediatric Department, Nanyang Second General Hospital, Nanyang, Henan, 473012, China
ABSTRACT
Objective: To compare the active ingredient content of Nanwuweizi (Kadsura longepedunculata)
and Beiwuweizi (Fructus Schisandrae Chinensis) collected in different seasons.
Methods: Microwave-assisted extraction was applied to extract the active ingredients
in Nanwuweizi and Beiwuweizi, with methanol as the solvent. High performance liquid
chromatography-diode array detection (HPLC-DAD) method was used to analyze the content
of each ingredient, with chromatographic conditions as follows: Brava BDS C18 column (250
mm×4.6 mm, 5 μm); column temperature: 30 ℃ ; mobile phase: water (A) - acetonitrile (B); gradient
elution: 0.8 mL/min; and detection wavelength: 225 nm.
Results: All ingredients waiting for detection reached their peaks within 75 min, with excellent
degree of separation and higher number of theoretical plates. Standard cu
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