孪蛋白与真核生物DNA复制起始-中国细胞生物学学报.PDF

中国细胞生物学学报 Chinese Journal of Cell Biology 2015, 37(12)﹕ 1692–1701 DOI﹕ 10.11844/cjcb.2015.12.0252 孪蛋白与真核生物DNA复制起始 ＃ ＃ ＃ * 代尚昆 贺晓艳 刘硕硕 邓 磊 王碧莹 范丽菲 ( 内蒙古大学生命科学学院, 呼和浩特 010021) 摘要 真核生物DNA复制起始受多种协同机制的严格调控, 以保证DNA复制起始在每个细 胞周期内仅发生一次。其中, 孪蛋白(Geminin)介导的一系列对DNA复制起始抑制的机制为高等真 报 核生物所特有, 对真核生物遗传物质忠实、稳定的传递至关重要。该文以孪蛋白与真核生物DNA 复制起始的关系为核心, 简要介绍了真核生物DNA复制起始的过程以及孪蛋白的结构、时空调控, 详细论述了目前发现的孪蛋白参与真核生物DNA复制起始调控的途径及其机制, 对前人的研究成 果进行了总结, 并提出了一些关于未来孪蛋白研究方向的思考。学 关键词 孪蛋白; DNA复制起始; 调控途径; 分子机制 Geminin and Eukaryotic DNA Replication Initiation 学 # # # Dai Shangkun , He Xiaoyan , Liu Shuoshuo , Deng Lei, Wang Biying, Fan Lifei* (School of Life Sciences, Inner Mongolia University, Hohhot 010021, China) 物 Abstract To ensure the initiation of DNA replication occurs only once per cell cycle, the initiation of DNA replication of eukaryotes is strictly regulated by multi-cooperative mechanisms. One mechanism is the Geminin mediated inhibition of DNA replication initiation, which is limited to higher eukaryotes and is vitally important for the faithful and stable transition of genetic material. Here, focusing on the relationship between Geminin and eukaryotic DNA replication 生 initiation, we simply introduce the process of eukaryotic DNA replication initiation and the structure, spatio-temporal regulation of Geminin. Additionally, we detailedly discuss the way and mechanism of how Geminin regulates or inhibits eukaryotic DNA replication in