分子生物学重点（Molecular biology focus）.docVIP

分子生物学重点（Molecular biology focus） pharmaceutical molecular biology Introduction Gene diagnosis: the application of molecular biology techniques to detect changes in the structure or expression of certain human genes, or to detect the presence of pathogens in the human genome, so as to achieve the purpose of diagnosis or monitoring efficacy Gene therapy: using molecular biology technique, specific, close or reduce the abnormal expression of genes; or normal exogenous genes in specific target cells to compensate for defects or a particular gene; gene into somatic expression of a protein factor specific therapeutic effect on the disease. Pharmacogenomics: To study the effect of genetic variation on efficacy and toxicity of drugs, drug development, promote open field development and rational use to strengthen the study of preclinical and clinical pharmacology and have an important impact on pharmaceutical economics. Chapter 1 molecular structures, properties and functions of nucleic acids DNA double helix structure The DNA molecule is composed of two complementary polynucleotide chains. The two chains, circling at the same axis in a certain space distance, form a double helix structure. DNA double chains are antiparallel. A chain runs from 5 to 3, and the other chain moves from 3 to 5. A = T, G = C The distances between pairs of bases are 3.4, with a distance of 34 for each pitch, including 10 pairs of bases. The law center. DNA is a template for self replication DNA transfers genetic information to intermediate substances by transcription, RNA RNA translates genetic information into proteins through translation In some viruses, RNA can replicate itself, and in certain viral protein synthesis, RNA can synthesize DNA under reverse transcriptase The structure and function of DNA Primary structure: the connection and arrangement of deoxyribonucleic acid in DNA molecules are the root cause of differences among species 1 encodes information about the primary structure of RNA