各种培养基配方（Various media formulations）.docVIP

各种培养基配方（Various media formulations） 1. nutrient broth Ingredients: peptone 10g, beef extract 3G, sodium chloride 5g, distilled water 1000mL, pH7.4. Method of preparation: according to the above ingredients mixed, dissolved after correction pH, 121 degrees high pressure sterilization 15min. 2. nutrient agar medium Ingredients: peptone 10g, beef extract 3G, sodium chloride 5g, agar 17g, distilled water 1000mL, pH7.2. Method of manufacture: dissolving all the components except agar in distilled water, correcting pH, adding agar, separating in the flask, 121 DEG C, 15min high pressure sterilizatio