固相萃取-福林酚比色法测定食用油中油溶性茶多酚-食品安全质量.PDF

5 4 Vol. 5 No. 4 2014 4 Journal of Food Safety and Quality Apr. , 2014 - 秦爱霞, 纪姝晶, 高 哲, 赫贝贝, 康小虎, 贾亚楠, 陈 灿, 崔 同* (, 071000) 摘 要: 目的 方法 , , , , 结果 Y=0.0007X -0.0381, R²=0.9566, 82.7% 84.8%, 5%, 结论 , , , 关键词: ; ; ; Determination of oil-soluble tea polyphenols in oil by solid phase extraction and Folin-Ciocalteu colorimetry * QIN Ai-Xia, JI Shu-Jing, GAO Zhe, HE Bei-Bei, KANG Xiao-Hu, JIA Ya-Nan, CHEN Can, CUI Tong (College of Food Science and Technology, Agricultural University of Hebei , Baoding 071000, China) ABSTRACT: Objective To establish a simple and accurate method for determination of oil-soluble tea po- lyphenols. Methods The oil-soluble tea polyphenols in oil were extracted by solid phase extraction column, and then eluted by acid methanol. The oil-soluble tea polyphenols were transformed into water-soluble tea po- lyphenols by saponification, and then determined by Folin-Ciocalteu colorimetry. Results The linear equation of this method was Y=0.0007X -0.0381, R²=0.9566. The recoveries were 82.7%∼84.8% and the relative standard deviations (RSDs) were about 5%, which can satisfied the routine analysis. Conclusion This method is sim- ple, and need no complex analysis instrument. The analysis results are reliable and it can be used for the deter- mination of