消食及胃冲剂对胃肠运动障碍小鼠胃肠运动影响实验探究.docVIP

消食及胃冲剂对胃肠运动障碍小鼠胃肠运动影响实验探究[摘要] 目的:观察消食和胃冲剂对胃肠运动障碍小鼠胃肠动力的作用。方法:以L-精氨酸制造胃肠运动障碍小鼠模型,采用营养性半固体糊灌胃法观察服用莫沙必利?消食和胃冲剂前后模型小鼠的小肠推进比和胃排空率的变化。结果:消食和胃冲剂有改善胃肠运动障碍小鼠胃肠动力的作用,与莫沙必利组作用相近(P0.05),两者对胃肠动力的恢复作用接近空白对照组(P0.05)。结论:消食和胃冲剂能有效改善胃肠运动障碍小鼠的胃肠动力。 [关键词] 消食和胃冲剂;胃肠运动障碍小鼠;胃排空;小肠推进 [中图分类号] R-332[文献标识码]A [文章编号]1673-7210(2010)04(a)-039-02 Experimental study of Xiaoshihewei Granules on the gastrointestinal motility in mice with gastrointestinal motility disorders CHI Peng1, LI Yan2 (1.Department of Gastroenterology, Anshan Central Hospital, Anshan 114000, China; 2.Department of Gastroenterology,Shengjing Hospital of China Medical University, Shenyang 110004, China) [Abstract] Objective: To observe the effect of Xiaoshihewei Granules on the gastrointestinal motility in mice with gastrointestinal motility disorders. Methods: Mouse models of gastrointestinal motility disorders were established with L-arginine, nutritional semisolid paste was used to observe the change of ratio and gastric emptying rate. Results: Xiaoshihewei Granules could improve the gastrointestinal motility in mice, and had similar effect with Mosapride group (P0.05), the recovery of gastrointestinal motility was close to the blank control group (P0.05). Conclusion: Xiaoshihewei Granules can effectively improve the gastrointestinal motility in mice with gastrointestinal motility disorders. [Key words] Xiaoshihewei Granules; Mice with gastrointestinal motility disorders; Emptying; Intestine advance 功能性消化不良是临床上常见的消化系统疾病,其中24%～78%的患者存在胃肠动力障碍。许多研究表明,中药有促胃肠动力的作用。本研究中消食和胃冲剂是在经典方剂保和丸的基础上加减而得,在前期的动物实验中已证实其对正常小鼠的胃排空和小肠推进起到明显的促进作用。本研究在此基础上进一步观察消食和胃冲剂对L-精氨酸所致胃肠运动障碍小鼠胃肠动力的改善作用。 1 材料与方法 1.1 主要药物与试剂 1.1.1 消食和胃冲剂的制备按《中国药典》标准收集焦山楂、神曲、茯苓、炒莱菔子、砂仁、炒枳实、炙甘草,以上药材均经鉴定为正品。将莱菔子、砂仁、炒枳实提取挥发油备用;焦山楂、茯苓、炙甘草与提油后的药渣混合,用水煎煮2次,煎煮液与提油时余下的药液合并,浓缩成干膏;将神曲研成细粉与干膏混匀,干燥;将混合物粉碎后均匀地喷洒上挥发油,即制成消食和胃冲剂。 1.1.2 营养性半固体糊的制备[1]10 g羧甲基纤维素钠溶于250 ml蒸馏水中,然后分别加入16 g奶粉、8 g糖、8 g淀粉,每加一次搅拌均匀,最后加入4 ml碳素墨水,配成300 ml约300 g的半固体糊状物,置于冰箱中冷藏保存,用前2 h取出,恢复至室