瑞香狼毒水提物小鼠血清对人膀胱癌T24细胞增殖影响.docVIP

瑞香狼毒水提物小鼠血清对人膀胱癌T24细胞增殖影响[摘要] 目的:探讨瑞香狼毒水提物(SCLA)的抗癌作用及机制。方法:以不同剂量SCLA灌胃给药后,于不同时间采集小鼠血清,处理体外培养的人膀胱癌T24细胞,用MTS比色法,于酶标仪上测定吸光度值,观察SCLA对细胞增殖的影响。结果:SCLA小鼠血清显著降低T24细胞增殖。结论:瑞香狼毒抑制人膀胱癌T24细胞增殖,可能成为膀胱癌新的治疗药物。 [关键词] 瑞香狼毒;膀胱癌T24细胞;细胞增殖 [中图分类号] R285[文献标识码]A [文章编号]1673-7210(2010)06(a)-016-02 Influence of SCLA mice serum on human bladder cancer T24 cell proliferation MAO Gang1, WANG Jian2*, CHEN Guojun2 (1.Medical College of Qinghai University, Xining 810001, China; 2.Department of Urinary Surgery, the Affiliated Hospital of Qinghai University, Xining 810000, China) [Abstract] Objective: To discuss the anti-cancer effect and its mechanism of SCLA. Methods: Mice serum was collected in different times after SCLA with different dosages was filled into stomachs, human bladder cancer T24 cells which were cultured in vitro were processed, MTS assay method was used, the light absorbance values were measured in ELISA reader, the influence of SCLA on cell proliferation was observed. Results: SCLA mice serum could prominently lower T24 cell proliferation. Conclusion: SCLA can inhibit human bladder cancer T24 cell proliferation and may become a new treatment for bladder cancer. [Key words] Stellera chamaejasme; Bladder cancer T24 cells; Cell proliferation 瑞香狼毒(Stellera chamaejasme L.SC)又名断肠草,为瑞香科狼毒属植物,分布于我国西北、华北等地。近几年研究表明,瑞香狼毒水提物(SCLA)小鼠血清对K562细胞、小鼠白血病L1210细胞和人胃癌SGC-7901细胞有显著的抑制增殖作用,但瑞香狼毒对泌尿系统、男性生殖系统肿瘤如膀胱癌细胞的抗肿瘤研究少有报道。本文采用血清药理学方法,探讨SCLA对体外培养的人膀胱癌T24肿瘤细胞增殖的影响。 1 材料与方法 1.1 材料 1.1.1 实验动物Balb/c小鼠,购自青海省地方病研究所,18～22 g,2～3月龄,SPF级,雌雄兼用。 1.1.2 SCLA的制备SC购自青海医药公司。将SC 200 g加水400 ml煮沸20 min 2次,上清液混合后过滤,制成口服液,每毫升含生药1.0 g。 1.1.3 细胞系膀胱肿瘤T24细胞购自西安交通大学第一附属医院泌尿外科实验室,用RPMI-1640培养液(10%胎牛血清、100 kU/L青霉素、100 mg/L链霉素),37℃、5% CO2培养,0.25%胰蛋白酶消化传代。 1.1.4 主要试剂RPMI-1640为赛默飞世尔生物化学制品(北京)有限公司产品;MTS为普洛麦格(北京)生物技术有限公司产品;胎牛血清为杭州四季青生物工程材料有限公司产品;丝裂霉素为日本协和发酵工业株式会社富士工厂产品。 1.1.5 主要设备超净工作台,相差倒置显微镜,二氧化碳恒温培养箱,水浴箱,水平离心机,全自动ELX800酶联免疫分析仪。 1.2 方法 1.2.1 药物血清制备Balb/c小鼠随机分成7组,禁食12 h后,分别灌胃给药,设