大鼠体外膜肺氧合模型的建立.pdfVIP

大鼠体外膜肺氧合模型的建立 秦科 张素斌 孙煦勇 梁东科 赖彦华 董建辉 [摘要] 目的 建立大鼠体外膜肺氧合(ECMO)模型。方法 选用成年健康雄性SPF级SD 大鼠，利用特制微型动物膜式氧合器，经右颈静脉插管引流，尾动脉插管灌注建立小预充量的 ECMO，转流时间120min，股动脉插管监测血流动力学指标及血气、电解质的变化。结果 90％大 鼠顺利建立ECMO模型，与ECMO前比较，平均动脉压(MAP)、红细胞压积(Hct)在ECMO30min、 ECMO60min、ECMO120min下降 （P＜0.05或P＜0.01），动脉血气及电解质指标差异无统计学意义 （P＞0.05），基本达到理想的ECMO标准。停机后呼吸和心血管循环功能顺利恢复，获得了 24h的较长时间生存。结论 本方法建立的大鼠ECMO模型切实可行，简单实用、重复性好。 体外循环；体外膜肺氧合；动物模型；大鼠 Establishmentofratmodelofextracorporealmembraneoxygenation QINKe ZHANGSu-bin SUNXu-yongLIANGDong-keLAIYan-huaDONGJian-hui DepartmentofOrganTransplantationof No.303HospitalofPeoplesLiberationArmy,Nanning530021,China Objective Toestablishaclinicallyrelevantratextracorporealmembraneoxygenation (ECMO)modelthatwouldbeavaluabletoolforinvestigatingpathophysiologicalandtherapeuticstrategies onbypass.Methods 20maleSDagedratswereanesthetizedandintubated.Therightexternaljugular vein,femoralarteryandtailarterywerecannulated.Thebloodwasdrainedfromtherightatriumviathe rightjugularveinandfurthertransferredbyasmallrollerpumptoaminiaturizedhollowfiberoxygenator andreturntothesystemiccirculationthroughthetailartery.ECMOwereperformedfor120minataflow rateof80to120ml/(kg ·min).Duringthewholeprocess,thehemodynamics,electrocardiograph(ECG) andblood-gasanalysiswereobserved.Results 18ofthe20animalswereperformedsuccessfully.Com paredwithbeforeECMO,MAPandHctweredecreasedafterECMO(P＜0.05orP＜0.01).Theblood-gas analysisatdifferenttimepointswerewithinanormalrange.AfterECMOweaning,cardiovascularandrespir atoryfunctionsrecoveredgradually.Conclusion Wehavesuccessfullyestablishedaminiaturecircuitcon sistingofprimeforaratECMOmodelthatmaintainsclinicallyacceptableresultsregardinghemodynamic parameters,bloodgases,andhemodilution.Thismodelwouldbevaluableforfurtheruseinclinicallyrele vantresearchstudies. Cardiopulmonarybypass; Extracorporealmembraneoxygenation;Animalmodel; Rats 10.3760/cma.j.issn.1001-9030.2011.12.077 基金项目：广西壮族自治区主席科技基金资助项目(1