antagonistic regulation, yet synergistic defense effect of bergapten and protease inhibitor on development of cowpea bruchid callosobruchus maculatus敌对的规定,然而协同防御bergapten和蛋白酶抑制剂对发展的影响豇豆bruchid callosobruchus maculatus.pdfVIP

Antagonistic Regulation, Yet Synergistic Defense: Effect of Bergapten and Protease Inhibitor on Development of Cowpea Bruchid Callosobruchus maculatus 1.¤a 1. 1,2 1¤b 2 3,4 Fengguang Guo , Jiaxin Lei , Yucheng Sun , Yong Hun Chi , Feng Ge , Bhimanagouda S. Patil , 3,4 5 1,4 Hisashi Koiwa , Rensen Zeng , Keyan Zhu-Salzman * 1 Department of Entomology, Texas AM University, College Station, Texas, United States of America, 2 State Key Laboratory of Integrated Management of Pest and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, People’s Republic of China, 3 Department of Horticultural Sciences, Texas AM University, College Station, Texas, United States of America, 4 Vegetable Fruit Improvement Center, Texas AM University, College Station, Texas, United States of America, 5 State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, People’s Republic of China Abstract The furanocoumarin compound bergapten is a plant secondary metabolite that has anti-insect function. When incorporated into artificial diet, it retarded cowpea bruchid development, decreased fecundity, and caused mortality at a sufficient dose. cDNA microarray analysis indicated that cowpea bruchid altered expression of 543 midgut genes in response to dietary bergapten. Among these bergapten-regulated genes, 225 have known functions; for instance, those encoding proteins related to nutrient transport and metabolism, development, detoxification, defense and various cellular functions. Such differential gene