arabidopsis hda6 regulates locus-directed heterochromatin silencing in cooperation with met1拟南芥hda6调节locus-directed异染色质沉默与met1合作.pdfVIP

Arabidopsis HDA6 Regulates Locus-Directed Heterochromatin Silencing in Cooperation with MET1 1,2. 1. 1 1 1 Taiko Kim To , Jong-Myong Kim , Akihiro Matsui , Yukio Kurihara , Taeko Morosawa , Junko 1 1 3 4 3 5 Ishida , Maho Tanaka , Takaho Endo , Tetsuji Kakutani , Tetsuro Toyoda , Hiroshi Kimura , Shigeyuki 2 6 1,7 Yokoyama , Kazuo Shinozaki , Motoaki Seki * 1 Plant Genomic Network Research Team, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan, 2 Graduate School of Science, The University of Tokyo, Tokyo, Japan, 3 Bioinformatics and Systems Engineering Division, RIKEN Yokohama Institute, Yokohama, Kanagawa, Japan, 4 Department of Integrated Genetics, National Institute of Genetics, Mishima, Shizuoka, Japan, 5 Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan, 6 Gene Discovery Research Group, RIKEN Plant Science Center, Yokohama, Kanagawa, Japan, 7 Kihara Institute for Biological Research, Yokohama City University, Yokohama, Kanagawa, Japan Abstract Heterochromatin silencing is pivotal for genome stability in eukaryotes. In Arabidopsis, a plant-specific mechanism called RNA–directed DNA methylation (RdDM) is involved in heterochromatin silencing. Histone deacetylase HDA6 has been identified as a component of such machineries; however, its endogenous targets and the silencing mechanisms have not been analyzed globally. In this study, we investigated the silencing mechanism mediated by HDA6. Genome-wide transcript profiling revealed that the loci silenced by HD