asynchronous replication and autosome-pair non-equivalence in human embryonic stem cells异步复制和autosome-pair异在人类胚胎干细胞.pdfVIP

Asynchronous Replication and Autosome-Pair Non- Equivalence in Human Embryonic Stem Cells ¤ Devkanya Dutta, Alexander W. Ensminger , Jacob P. Zucker, Andrew Chess* Center For Human Genetic Research and Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Simches Research Center, Boston, Massachusetts, United States of America Abstract A number of mammalian genes exhibit the unusual properties of random monoallelic expression and random asynchronous replication. Such exceptional genes include genes subject to X inactivation and autosomal genes including odorant receptors, immunoglobulins, interleukins, pheromone receptors, and p120 catenin. In differentiated cells, random asynchronous replication of interspersed autosomal genes is coordinated at the whole chromosome level, indicative of chromosome-pair non-equivalence. Here we have investigated the replication pattern of the random asynchronously replicating genes in undifferentiated human embryonic stem cells, using fluorescence in situ hybridization based assay. We show that allele-specific replication of X-linked genes and random monoallelic autosomal genes occur in human embryonic stem cells. The direction of replication is coordinated at the whole chromosome level and can cross the centromere, indicating the existence of autosome-pair non-equivalence in human embryonic stem cells. These results suggest that epigenetic mechanism(s) that randomly distinguish between two parental alleles are emerging in the cells of the inner cell mass, the source of human embryonic stem cells. Citation: Dutta D, Ensminger AW, Zucker JP, Chess A (2009) Asynchronous Replication and Autosome-Pair Non-Eq