athkt1;1 mediates nernstian sodium channel transport properties in arabidopsis root stelar cellsathkt1;1介导nernstian在拟南芥根stelar细胞钠通道传输特性.pdfVIP

AtHKT1;1 Mediates Nernstian Sodium Channel Transport Properties in Arabidopsis Root Stelar Cells 1¤a 1¤b 1,3 2 2 1¤c Shaowu Xue , Xuan Yao , Wei Luo , Deepa Jha , Mark Tester , Tomoaki Horie , Julian I. Schroeder1* 1 Division of Biological Sciences, Cell and Developmental Biology Section, University of California, San Diego, La Jolla, California, United States of America, 2 Australian Centre for Plant Functional Genomics, University of Adelaide, South Australia, Australia, 3 State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China Abstract + The Arabidopsis AtHKT1;1 protein was identified as a sodium (Na ) transporter by heterologous expression in Xenopus laevis oocytes and Saccharomyces cerevisiae. However, direct comparative in vivo electrophysiological analyses of a plant HKT transporter in wild-type and hkt loss-of-function mutants has not yet been reported and it has been recently argued that heterologous expression systems may alter properties of plant transporters, including HKT transporters. In this report, we analyze several key functions of AtHKT1;1-mediated ion currents in their native root stelar cells, including Na+ and K+ conductances, AtHKT1;1-mediated outward currents, and shifts in reversal potentials in the presence of defined intracellular and extracellular salt concentrations. Enhancer trap Arabidopsis plants with GFP-labeled root stelar cells were used to investigate AtHKT1;1-dependent ion transport properties using patch cla