bone marrow derived mesenchymal stem cells incorporate into the prostate during regrowth骨髓提取间充质干细胞在再生纳入前列腺.pdfVIP

Bone Marrow Derived Mesenchymal Stem Cells Incorporate into the Prostate during Regrowth 1 1,2 3 2 1,2,4 Veronica R. Placencio , Xiaohong Li , Taylor P. Sherrill , Gloria Fritz , Neil A. Bhowmick * 1 Department of Cancer Biology, Vanderbilt University, Nashville, Tennessee, United States of America, 2 Department of Urologic Surgery, Vanderbilt University, Nashville, Tennessee, United States of America, 3 Department of Pulmonary and Critical Care Medicine, Vanderbilt University, Nashville, Tennessee, United States of America, 4 Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, California, United States of America Abstract Background: Prostate cancer recurrence involves increased growth of cancer epithelial cells, as androgen dependent prostate cancer progresses to castrate resistant prostate cancer (CRPC) following initial therapy. Understanding CRPC prostate regrowth will provide opportunities for new cancer therapies to treat advanced disease. Methodology/Principal Findings: Elevated chemokine expression in the prostate stroma of a castrate resistant mouse model, Tgfbr2fspKO, prompted us to look at the involvement of bone marrow derived cells (BMDCs) in prostate regrowth. We identified bone marrow cells recruited to the prostate in GFP-chimeric mice. A dramatic increase in BMDC recruitment for prostate regrowth occurred three days after exogenous testosterone implantation. Recruitment led to incorporation of BMDCs within the prostate epithelia. Immunofluorescence staining suggested BMDCs in the prostate coexpressed androgen receptor; p63, a bas