cannabinoid receptor 2 signaling does not modulate atherogenesis in mice大麻素受体2信号不调节小鼠的血管硬化.pdfVIP

Cannabinoid Receptor 2 Signaling Does Not Modulate Atherogenesis in Mice 1. 1. 1 1 Florian Willecke , Katharina Zeschky , Alexandra Ortiz Rodriguez , Christian Colberg , Volker ¨ 2 2 2 1 1 1 Auwarter , Stefan Kneisel , Melanie Hutter , Andrey Lozhkin , Natalie Hoppe , Dennis Wolf , Constantin ¨ 1 1 1 1 1 von zur Muhlen , Martin Moser , Ingo Hilgendorf , Christoph Bode , Andreas Zirlik * 1 Department of Cardiology, University of Freiburg, Freiburg, Germany, 2 Forensic Toxicology, Institute of Forensic Medicine, University Medical Center Freiburg, Freiburg, Germany Abstract Background: Strong evidence supports a protective role of the cannabinoid receptor 2 (CB ) in inflammation and 2 atherosclerosis. However, direct proof of its involvement in lesion formation is lacking. Therefore, the present study aimed to characterize the role of the CB2 receptor in Murine atherogenesis. Methods and Findings: Low density lipoprotein receptor-deficient (LDLR2/ 2) mice subjected to intraperitoneal injections of the selective CB2 receptor agonist JWH-133 or vehicle three times per week consumed high cholesterol diet (HCD) for 16 weeks. Surprisingly, intimal lesion size did not differ between both groups in sections of the aortic roots and arches, suggesting that CB2 activation does not modulate atherogenesis in vivo