cell-free microrna expression profiles in malignant effusion associated with patient survival in non-small cell lung cancermicrorna表达谱游离在恶性积液与非小细胞肺癌患者生存有关.pdfVIP

Cell-Free MicroRNA Expression Profiles in Malignant Effusion Associated with Patient Survival in Non-Small Cell Lung Cancer 1,2 1 1 1 1 1 2 Tingting Wang , Mingming Lv , Sunan Shen , Sheng Zhou , Ping Wang , Yueqiu Chen , Baorui Liu , 3 1 Like Yu , Yayi Hou * 1 Immunology and Reproduction Biology Lab, Medical School State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Key Laboratory of Molecular Medicine, Nanjing University, Nanjing, Jiangsu, China, 2 Department of Oncology, Drum Tower Hospital Affiliated to Medical School of Nanjing University and Clinical Cancer Institute of Nanjing University, Nanjing, Jiangsu, China, 3 First Department of Respiratory Medicine, Nanjing Chest Hospital, Nanjing, Jiangsu, China Abstract Objective: MicroRNAs (miRNAs) expression is altered in cancer cells, and miRNAs could serve as diagnostic and prognostic biomarker for cancer patients. This study was designed to analyze circulating miRNAs expression in the malignant pleural effusion (MPE) and their association with patient survival in non-small cell lung cancer (NSCLC). Methods: Pleural effusion from 184 patients with NSCLC and MPE were collected. MiRNA microarray and bioinformatics interpretation were used to evaluate miRNA expression profiles in 10 NSCLC patients with different survival prognosis. Associations were validated in 184 patients (randomly classified into training and validation set with equal number in each group) using quantitative RT-PCR. Risk scores were formulated based on the expression signature of miRNAs. Clinical data, such as patient survival, were collected for correlation analysis. Results: Thirty-three miRNAs were found to be altered more than two